nadB [2019-08-08 09:21:18]

L-aspartate oxidase

locus: BSU_27870

pI: 6.45

mw: 58.07 kDa

protein length: 531 aa

gene length: 1596 bp

function: NAD biosynthesis

product: L-aspartate oxidase

essential: no

ec: 1.4.3.16

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 2,847,871 → 2,849,466

The protein

Catalyzed reaction/ biological activity

• L-aspartate + O₂ = iminosuccinate + H₂O₂ (according to Swiss-Prot)

Protein family

• FAD-dependent oxidoreductase 2 family (with [protein|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|SdhA], according to UniProt)

Modification

• phosphorylated on Arg-104 [Pubmed|22517742]

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|5KXJ] (from Salmonella typhimurium, 38% identity)

Expression and Regulation

Operons

• genes: [gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]-[gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]-[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]
  description: [Pubmed|8444804]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8444804], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|510DE075EA964D22C42DEB171509F6CDA600A402|NadR]: repression, [Pubmed|16199587], in [regulon|510DE075EA964D22C42DEB171509F6CDA600A402|NadR regulon]

  regulation

  • repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|16199587]

  additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|nadB]' [PubMed|20525796]

Biological materials

Mutant

• BKE27870 (Δ[gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGCCATCCTCCTGTTG, downstream forward: _UP4_AGAAAAAACGAGGGGATTTG
• BKK27870 (Δ[gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGCCATCCTCCTGTTG, downstream forward: _UP4_AGAAAAAACGAGGGGATTTG

labs

• [SW|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]

References

• 8444804,18959769,16199587,20525796,22517742